P-GSE21866-7 - P-GSE21866-7

growth protocol
The tongue squamous cell carcinoma cell line (LK0412) was derived and cultured under the same serum-free epithelial medium with high levels of amino acids (EMHA) as the normal cells (Roberg, K et al. Differentiation, 76:868-80, 2008 and Grafström, R.C., Culture of Epithelial cells, 2nd ed, John Wiley and Sonc Inc, NY, 2002). Cultures were transferred on regulat tissue culture plastic at 100% confluency and cells in passage 14-18 were used in the experiments.
Experiment E-GEOD-21866