P-GSE51454-3 - P-GSE51454-3

growth protocol
We used human lymphoblastoid primary cultures and two human lymphoblastoid cell lines, independently immortalized with Epstein-Barr virus (catalog No. VR-1492; ATCC, Manassas, VA). All cell cultures were propagated in RPMI media supplemented with 10% fetal bovine serum (FBS), L-glutammine 2 mM and antiobiotics (10 000 U/ml penicillin and 10 mg/ml streptomycin) (Invitrogen Calrsbad, CA) at 37ºC in an atmosphere of 5% CO2. In the case of primary cultures, 1% phytohemaglutinine were added
Experiment E-GEOD-51454