P-MTAB-35603 - P-MTAB-35603
nucleic acid extraction protocol
Total RNA was extracted using Invitrogen's Trizol reagent. Quality was assessed by gel electrophoresis and spectrophotometry (using a Nanodrop spectrophotometer). cDNA was synthesised using an anchored poly(dT) primer (20mer) and INvitrogen's SuperscriptII reverse transcriptase. RNA was hydrolysed in basic conditions, and cDNA measured using a Nanodrop sprectrophotometer. About 2.5ug of cDNA were sent to Nimblegen for labelling (Cy3) and hybridisation using their own protocols.