P-GSE49614-2 - P-GSE49614-2
nucleic acid extraction protocol
Total DNA was extracted using Qiagen Dneasy Blood e Tissue Kit, following manufacturer's instructions. DNA purity was checked by spectrophotometer, and DNA integrity by examination on agarose gel electrophoresis. DNA concentration was measured with the Quant-IT dsDNA BR assay kits with the Qubit fluorometer (Invitrogen) following manufacturer’s instructions.