P-GSE40140-12 - P-GSE40140-12
Experiments were carried out on Arabidopsis thaliana ecotype Columbia-0. Two T-DNA insertion mutant lines of WRKY22, wrky22-ko1 (SALK_047120) and wrky22-ko2 (SALK_098205), were obtained from the Arabidopsis Biological Resource Center, Ohio State University, USA. All seeds were surface-sterilized with 0.5% of sodium hypochloride for 20 minutes and washed at least five times with sterilized water. Seeds were sown on plates with 0.55% of phytagel (Sigma-Aldrich, St. Louis, MO, USA) in half-strength MS medium (Duchefa Biochemie BV, Haarlem, Netherlands) containing 0.5% sucrose at pH 5.7, and kept at 4oC in the dark for 3 days to achieve uniform germination. The plates were then transferred to a growth chamber and placed vertically, and plants were grown at 22ºC under a 16-hour light (81 μmol s-1 m-2)/8-hour dark cycle until 9-day-old.