P-GSE40140-6 - P-GSE40140-6

labelling protocol
One hundred pg of DNA was amplified using the GenomePlex Whole Genome Amplification Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions. The amplified DNA was then purified using the QIAquick PCR cleanup kit (Qiagen). The purified DNA was quantified using a Nanodrop spectrophotometer and labeled with Cy3 (for Columbia) and Cy5 (for WRKY22-18×c-myc in wrky22-ko2) fluorescent dyes. Labeling experiments were performed at the DNA Microarray Core facility, Institute of Plant and Microbial Biology, Academia Sinica, as described on
Experiment E-GEOD-40140