P-GSE39151-2 - P-GSE39151-2
Muscle larvae (ML) of T. spiralis (strain ISS534) were obtained from rats 35 days post infection by digestion of minced skeletal muscle in 1% pepsin, 1% HCl for 3 h at 37°C with agitation as previously described [PMID:840494]. To purify adult worms and newborn larvae, Wistar rats at 6 weeks of age were inoculated with T. spiralis (strain ISS534) orally with a dose of 8000 larva per rat. At 30 h or 6 days post infection, all rats were executed and the entire intestines were removed, opened longitudinally and cut into pieces (about 0.5-1 cm). The fragmented intestine was put on a layer of gauze which was immersed into 37 °C 0.9% sodium chloride solution and incubated for 3 h. Adult T. spiralis worms would migrate into the liquid phase, which were harvested by centrifugation. To obtain new-born larvae, adult worms collected at 6 days post infection were incubated in IMDM in 75-cm2 cell culture plate at 37 °C, the newborn larvae were harvested every 12 h.