P-GSE29378-2 - P-GSE29378-2
nucleic acid extraction protocol
Using a scalpel CA1 and CA3 subfields were dissected from sectioned but unlabeled tissue on dry ice and immediately placed into elution buffer for RNA extraction. Total RNA from each sample was isolated using the RNeasy Micro Kit with DNase I treatment (QIAGEN; Valencia, CA), then tested for quality on the Agilent 2100 Bioanalyzer using RNA 6000 Nano Chips (Agilent Technologies; Palo Alto, CA). This extraction followed the protocols provided by the manufacturers.