P-GSE28381-4 - P-GSE28381-4
nucleic acid extraction protocol
Type I collagen gels were digested with 5 mls of a 0.01% collagenase mixture containing RNase OUT in PBS at 4oC for approximately 1 hour on a rotator. Following two low speed (500 rpm) spins and PBS washes 1ml TRIZol reagent was added to each individual sample. Total RNA was isolated following the manufacturer's instructions. Total RNA was purified using the Qiagen RNeasy Kit according to the manufacturer's instructions. Due to limitations in yield, 4 separate RNA samples were pooled for application to each individual array.