P-MTAB-32857 - P-MTAB-32857
nucleic acid sequencing protocol
The emulsion PCRs (emPCRs) for the library titrations and the sequencing reactions were prepared according to the emPCR Method Manual - Lib-L LV for GS FLX+ Series - XL+. Sequencing was performed on a GS FLX+ Instrument following the Sequencing Method Manual for GS FLX+ Series / XL+ Kit. Sequencing images were analyzed with the Roche 454 Shotgun-pipeline program and then a FastQ file was generated.
454 GS FLX+