P-MTAB-28464 - P-MTAB-28464
nucleic acid sequencing protocol
Libraries were prepared following the Rapid Library Preparation Method Manual for GS FLX+ Series / XL+ Kit. The emulsion PCRs (emPCRs) for the library titrations and the sequencing reactions were prepared according to the emPCR Method Manual â Lib-L LV for GS FLX+ Series - XL+. Sequencing was performed on a GS FLX+ Instrument following the Sequencing Method Manual for GS FLX+ Series / XL+ Kit. Sequencing images were analyzed with the Roche 454 Shotgun-pipeline and Amplicons-pipeline programs. Two FastQ files were generated, one per lane.
454 GS FLX+