P-GSE46585-4 - P-GSE46585-4

labelling protocol
100 ng total RNA per sample was introduced into an RT-IVT reaction. Prior to RT-IVT, the total RNA samples were spiked with in-vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In-Mix, Agilent Technologies), which served as the internal labeling control for linearity, sensitivity and accuracy. The spiked total RNA was reverse transcribed into cDNA and then converted into labeled RNA by in-vitro transcription incorporating Cyanine-3-CTP, this was done using the Low Input Quick-Amp labeling Kit One-Color, Agilent Technologies.
Experiment E-GEOD-46585