P-GSE46585-5 - P-GSE46585-5

hybridization protocol
Cyanine-3-CTP, 1.65ug per sample was fragmentd and prepared for One-Color based hybridization using the Gene Expression Hybridization Kit, Agilent Technologies. cRNA samples were hybridized at 65˚C for 17 hrs on separate Agilent Chicken V2 4x44K Gene Expression Microarrays. The microarrays were washed with increasing stringency using Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (SIGMA).
Experiment E-GEOD-46585