P-GSE43298-11 - P-GSE43298-11
nucleic acid library construction protocol
AllPrep kit (Qiagen) RRBS was performed by Integragen SA (Evry, France), as described elsewhere (Gu et al., 2011; Smallwood et al., 2012). In brief, 300 ng of mouse genomic DNA was digested with 14 units of MspI (NEB). After end-repair, A-tailing and ligation to methylated Illumina adapters, the library fragments of 40-220 bp were gel isolated, subjected to a double bisulfite conversion with the EpiTect Bisulfite kit from Qiagen, PCR amplified and sequenced on an Illumina HiSeq2000 sequencer as paired-end 75-bp reads.