P-GSE32376-3 - P-GSE32376-3

labelling protocol
Briefly, 100 ng of total RNA was dephosphorylated with calf intestine alkaline phosphatase for 30 min at 37°C. Denaturation was performed by adding DMSO and incubating at 100°C for 7 min and immediately transferred to a ice water bath. Ligation was performed with pCp-Cy3 at 16 °C for 2 h. The labeled samples were dried completely in a vacuum concentrator and resuspended in 18 ul of nuclease free water.
Experiment E-GEOD-32376