P-GSE32376-4 - P-GSE32376-4

hybridization protocol
The hybridization mixture (10X GE blocking agent (4.5µl) and 2X Hi-RPM hybridization buffer (22.5µl)) along with the labelled miRNA sample was heated for 5 min at 100 °C and immediately cooled on ice. Each 45 µL sample was hybridized onto a microarray at 55 °C for 20 h. Slides were then washed for 5 min in GE wash buffer 1 at RT and again for 5 min in GE wash buffer 2 at 37 °C, followed by an acetonitrile wash for 1 min at RT to dry the slides completely.
Experiment E-GEOD-32376