P-GSE25260-2 - P-GSE25260-2
nucleic acid library construction protocol
By polyacrylamide gel electrophoresis, the small RNAs (~17-27 nt) were purified from 100 μg of total RNA and ligated to a 5’ RNA adapter and a 3’ RNA adapter. Reverse transcriptase reaction followed by low cycle PCR was performed to obtain sufficient product for SBS sequencing. PCR products were collected by gel purification and sequenced by Solexa technology.