P-GSE14462-4 - P-GSE14462-4
nucleic acid library construction protocol
The middle parts of the flag leaves were collected for RNA extraction at 08:00 on the day when the flag leaf was fully extended (record the length of the flag leafs each morning until the length did not increase any longer). The total RNAs were isolated the operation instruction of the Trizol Reagent (Invitrogen, USA, Cat. No.18068). The equivalent amounts of total RNAs from three biological replica were sampled and mixed together for the construction of autotriploid and diploid sRNA libraries as described by Lu et al. sRNA libraries were sequenced using Illumina Genome Analyzers as recommended by the manufacturer.