P-GSE45383-2 - P-GSE45383-2

Type
nucleic_acid_extraction
Description
RNA extraction and purification were performed as described (Bonawitz et al., 2008) from wild-type DBY2006 and rph1Δ cultures grown for 24 hours after addition of 50 μM menadione or ethanol. RNA quality bioanalysis, cDNA synthesis, and hybridization to a Roche/NimbleGen 12x135K array were performed in collaboration with the Yale University W.M. Keck Foundation Biotechnology Resource Laboratory according to the manufacturer’s protocol. Two independent biological replicates were prepared for each sample, and hybridization was performed in duplicate to generate technical replicates.
Links
Experiment E-GEOD-45383