P-GSE44942-3 - P-GSE44942-3

nucleic acid library construction protocol
Primary mouse dermal fibroblasts were cultured for 8 days. Either cell were treated with Thapsigargin to induce ER stress and induces ATF4 expression for 3 hours or left untreated. RNA was harvested using Trizol reagent from the primary cells. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment E-GEOD-44942