P-GSE40548-2 - P-GSE40548-2
nucleic acid library construction protocol
RNA was isolated using TRIzol-chloroform precipitation, followed by clean-up with the Rneasy kit (Qiagen) including DNA digestion. RNAseq was carried out using standard SOLiD and Illumina protocols. mRNA was enriched by ribosomal depletion (SOLiD) or poly-A selection (Illumina).