P-MTAB-30839 - P-MTAB-30839
The TRIzol lysates were processed according to the manufacturers instructions. Total RNA was isolated and purified using the RNeasy Micro kit (Qiagen, Hilden, Germany): 250 ul of ethanol was added to the upper aqueous phase of the processed TRIzol samples and directly transferred to the RNeasy spin columns for purification. RNA concentrations and OD 260/280 ratios were measured with the NanoDrop ND-1000 UV-VIS spectrophotometer (NanoDrop Technologies, Wilmington, USA). Assessment of total RNA quality and purity was performed with the RNA 6000 Nano assay on the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).