P-G1001-1 - P-G1001-1
We anesthetized male Sprague-Dawley (albino) rats (270–330 g) by intraperitoneal injection of a mixture of xylazine (13 mg/kg) and ketamine (87 mg/kg), then induced a retinal tear in both eyes of each rat. For this purpose, we used a 27-gauge needle to penetrate the pars plana, then scraped the superior temporal retina medially to laterally, as previously described (Mol Vision. 1998;4:3; Neurosci Lett. 2003;338:29–32; Br J Ophthalmol. 1996;80:458–464; J Comp Neurol. 1981;202:493–5). Care was taken to prevent lens and sclera damage. Control animals received no injury. All animals were killed by intraperitoneal injection of a mixture of xylazine (26 mg/kg) and ketamine (174 mg/kg). All protocols used in this study were approved by the Animal Care and Use Committee of the University of Tennessee Health Science Center and were in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.