P-CAGE-24529 - P-CAGE-24529
Labeled target cRNA was prepared according to the GeneChip Expression Analysis Technical Manual (701025 Rev. 3) of Arabidopsis Genome ATH1 DNA array (Affymetrix, Santa Clara, CA, USA). - Double-stranded cDNA was prepared from 12 ug of total RNA. - Double strand cDNA synthesis using SuperScript Choice System (Invitrogen, Carlsbad, CA, USA) - Synthesis of biotin-labeled cRNA through in vitro transcription using BioArray HighYield RNA Transcript Kit (Enzo, New York, NY, USA) - Purification labeled cRNA with RNeasy column (Qiagen, Hilden, Germany) - Fragmentation of labeled cRNA. 94 C, 35 min in Fragmentation Buffer (Affymetrix).
Amount of nucleic acid labeled, Amplification, Used Label