P-GSE20296-5 - P-GSE20296-5

Type
hybridization
Description
The slides were pretreated with 1% BSA(fraction V), 20 × SSC, 10% SDS (30 min at 50°C) and washed with 2 × SSC (3 min) and 0.2 × SSC (3 min) and hybridized overnight in cocktail containing 50 × Denhardt's, 20 × SSC, 10% SDS, 10μg/μl polyadenylate and 10 μg/μl yeast tRNA. All chemicals were from Sigma-Aldrich.
Links
Experiment E-GEOD-20296