P-EMBL-153 - P-EMBL-153
Yeast cells (30 ml of culture) were resuspended in 400 µl of TES buffer (10 mM Tris/HCl pH 7.5; 10 mM EDTA; 0.5% (w/v) SDS). Then samples were extracted twice with 400 µl of acidic phenol, then once with 400 µl of chloroform. Total RNA was then ethanol-precipitated, air-dried and resuspended in 100 µl of RNAse free water. This crude RNA fraction was further purified using the Qiagen RNeasy Mini Kit (RNA clean-up protocol) including the on-column DNase digestion protocol. Finally the RNA was ethanol-precipitated and stored at -80°C under 70% ethanol until use.
Amplification, Extracted product