P-MTAB-23644 - P-MTAB-23644

Human trabecular meshwork primary cells at passage 4, seeded on either 3 cm or 10 cm dishes, were grown to between 65-90% confluency, washed twice with PBS and exposed to recombinant adenoviruses in 1 ml or 3 ml serum-free medium respectively at multiplicity of infections (moi) ranging from 1.6 X103-1.6 X104 vg/cell. After exposure to the virus for 90 min, complete media was added and incubation continued for 48 h or 72 h.
Experiment E-MEXP-3427