P-MTAB-23643 - P-MTAB-23643

Human trabecular meshwork cells cells were scraped from tissue culture dishes with guanidine thiocyanate buffer (RLT, Qiagen). Total RNA was extracted by loading the solution onto a QIA ShredderTM column (Qiagen) and continued by the use of the RNeasy Mini kit with on-column RNase-free DNAse digestion according to manufacturer’s recommendations (Qiagen). Purified RNA was eluted in 30 ul RNase-free water and the concentration measured with a NanoDrop ND-100 spectrophotometer (Thermo Fisher Scientific). Total RNA recoveries averaged 65.4 ± 3.6 ug and 11.9 ± 1.1 ug per 10 and 3 cm culture dishes respectively. RNA quality was assessed by measuring the size distribution on an Agilent Bioanalyzer (Agilent Technologies). (Parameters: Extracted product = total_RNA, Amplification = none)
Experiment E-MEXP-3427