P-CVDE-2 - P-CVDE-2
direct fluorescent labelling of 2 ug amplified RNA, anneling of 0.5 ug Random Primer
and reverse-transcribed into cDNA with Superscript III reverse transcriptase for 1h
at 42 degrees C in the presence of 0.4 mM dGTP, 0.4 mM dATP, 0.4 mM dTTP, 0.24 mM dCTP,0.125 mM
Cy3-dCTP. After hydrolysis of RNA in 0.2 M NaOH, Cy3-labeled probes were purified with
Microcon YM-30 column.