cbil.upenn.edu:RAD.Protocol:1985:Protocol - cbil.upenn.edu:RAD.Protocol:1985:Protocol

Cells were lysed in a buffer containing guanidine isothiocyanate and beta-Mercaptoethanol. The lysate was homogenized and precipitated with 70% ethanol, transferred to a silica membrane column, and DNA and proteins were removed by a series of washes and centrifugations. Highly purified total RNA was then eluted from the column using RNase-free water. For the Nanoprep kit, 2 x 10 uL elutions were used. For the Microprep kit, 2 x 30 uL elutions were used.
All experiments using protocol cbil.upenn.edu:RAD.Protocol:1985:Protocol: (E-CBIL-32, E-CBIL-42, E-CBIL-43, E-CBIL-47)