cbil.upenn.edu:RAD.Protocol:4715:Protocol - cbil.upenn.edu:RAD.Protocol:4715:Protocol
The IP sample (~1ng) was made Blunt-Ended w/ T4 DNA Pol (IVGN) following the manufacturer notes (Incubate 12 degrees C 15 minutes, incubating at 37 can lead to 1bp overhangs), then purified with a Qiaquick Reaction Cleanup kit following the manufacturer notes. After Ligating the sample to an annealed oligo linker (OJW102 and OJW103) and purifying with a Qiaquick Cleanup column, a PCR reaction was performed. This reaction used the OJW102 oligo as a primer and is carried out for 15-20 cycles. The PCR is purified with the Qiagen kit as before an the entire procedure is repeated (second round) if necessary.
|All experiments using protocol cbil.upenn.edu:RAD.Protocol:4715:Protocol: (E-CBIL-36, E-CBIL-38)|