cbil.upenn.edu:RAD.Protocol:4778:Protocol - cbil.upenn.edu:RAD.Protocol:4778:Protocol
Chromatin was prepared from half of a mouse liver. 2 ug was precleared by incubating with protein-G agarose. 2 ug GR Antiserum (sc-1002 Santa Cruz) was added and the sample was incubated at 4 degrees C overnight. Isolated immunoprecipitates were washed extensively then eluted. Following reverse crosslinking and a proteinase K digestion, the DNA was isolated via phenol/chloroform extraction and an ethanol precipitation.