cbil.upenn.edu:RAD.Protocol:4761:Protocol - cbil.upenn.edu:RAD.Protocol:4761:Protocol

Slides are Pre-Hybridized in 5X-SSC, 0.1% SDS , 1% BSA at 42 C for 45 min. Rinsed 5X in deionized H20 and 1X in Isopropanol and dried by centrifugation for 1 min at 1000rpm. The DNA hybridization mix was combined with mouse Cot-1 DNA and incubated overnight at 42 C in a Corning hybridization chamber. Coverslip removed in 2X SSC, 0.1%SDS at room temp. Slide was washed once in 0.2X SSC, 0.1% SDS (42 C, 5 min, with agitation), once in 0.2X SSC (room temperature, 5 min, with agitation)and dried by centrifugation at 1000rpm for 3 min.
cot-1 DNA
All experiments using protocol cbil.upenn.edu:RAD.Protocol:4761:Protocol: (E-CBIL-36, E-CBIL-38)