cbil.upenn.edu:RAD.Protocol:3547:Protocol - cbil.upenn.edu:RAD.Protocol:3547:Protocol

The RNeasy procedure represents a novel technology for RNA isolation. This technology combines the selective binding properties of a silica-gel-based membrane with the speed of microspin technology. A specialized high-salt buffer system allows RNA to bind to the RNeasy silica-gel membrane. Biological samples are first lysed and homogenized in the presence of a highly denaturing guanidine isothiocyanate (GITC)-containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. Ethanol is added to provide appropriate binding conditions, and the sample is then applied to an RNeasy column where the total RNA binds to the membrane and contaminants are efficiently washed away. High-quality RNA is then eluted in water.
All experiments using protocol cbil.upenn.edu:RAD.Protocol:3547:Protocol: (E-CBIL-10, E-CBIL-24, E-CBIL-25, E-CBIL-36, E-CBIL-40, E-CBIL-44, E-CBIL-8, E-CBIL-9)