cbil.upenn.edu:RAD.Protocol:344:Protocol - cbil.upenn.edu:RAD.Protocol:344:Protocol

The filters were pre-hybridized for 6 h in 10 ml of hybridization solution (200 mM sodium phosphate, 10 mM EDTA, 1% BSA, 6.7% SDS and 6.7% deionized formamide). Radiolabeled probes were denatured at 95 C for 5 min and chilled on ice. The probes were added into 6 mL of fresh hybridization solution along with 50 ul Human Cot I DNA (GIBCO BRL Life Technologies, Rockville, MD), and the filters were hybridized overnight. Hybridizations were carried out at 62 C in a hybridization oven with continuous rotation. Filters were then washed three times (40 mM sodium phosphate solution, 1 mM EDTA, 1% SDS).
All experiments using protocol cbil.upenn.edu:RAD.Protocol:344:Protocol: (E-CBIL-17, E-CBIL-3)