cbil.upenn.edu:RAD.Protocol:1024:Protocol - cbil.upenn.edu:RAD.Protocol:1024:Protocol
RNA is amplified using the MessageAmp aRNA Kit (Ambion, Austin, TX). Total RNA is reverse transcribed using a T7 Oligo(dT) Primer and reverse transcriptase at 42C for 2 hours. Second-strand synthesis is performed using DNA Polymerase. The resulting cDNA is purified with a filter cartridge. Double-stranded cDNA is concentrated and in vitro transcribed at 37C to make antisense RNA (aRNA). The aRNA is DNase-treated, purified with a filter cartridge, and eluted with nuclease-free water.
|All experiments using protocol cbil.upenn.edu:RAD.Protocol:1024:Protocol: (E-CBIL-13, E-CBIL-37, E-CBIL-6, E-CBIL-7)|