P-MEXP-4211 - P-MEXP-4211
Labeled cDNA is done by using 5 ug of aRNA as template in A RT direct labeling reaction. aRNA was done using the MessageAmp aRNA kit from AMbion (cat 1750). LabelStar Qiagen kit was used (See protocol for Direct labeling of cDNA with Cyanine dyes. Reaction is primed with 2 micrograms of random nonamers, (SIGMA R7647) and labelled with 1ul of Cy5-dUTP (Amersham 25nmol tube, 1mM, PA55022).
Amount of nucleic acid labeled, Amplification, Label used
|All experiments using protocol P-MEXP-4211: (E-CAGE-28, E-CAGE-29, E-CAGE-30, E-CAGE-31, E-CAGE-32, E-CAGE-33)|