nucleic acid library construction protocol
The libraries were constructed according to manufacturer guidelines. (Illumina). Briefly, 350 to 900 ng of total RNA determined by Qbit high sensitivity spectrofluorometric measurement (Invitrogen) was poly-A selected and reverse transcribed using Illuminas TruSeq RNA library preparation kit V2. Each sample was fitted with a unique adapter containing a 6 base molecular barcode for high level multiplexing. Prior sequencing, a 12 cycles of PCR amplification was performed on the samples.