Investigation Title	Transcription profiling of skeletal muscle tissue from spontaneously hypertensive rats and normotensive rats in a set of recombinant inbred strains	
Comment[AdditionalFile:XLS]	E-TABM-458.hybs.xls
Comment[Submitted Name]	Gene Expression Profiling in Skeletal Muscles Tissue in Recombinant Inbred Rat Strains (BN,SHR,BNXSHR,SHRXBN)	
Experimental Design	in_vivo_design	strain_or_line_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF		mo	mo	EFO	
Comment[AEMIAMESCORE]	5	
Comment[ArrayExpressReleaseDate]	2008-04-15	
Comment[SecondaryAccession]		
Comment[ArrayExpressAccession]	E-TABM-458	
Comment[MAGETAB TimeStamp_Version]	2010-10-16 07:41:01 Last Changed Rev: 14677	
Experimental Factor Name	StrainOrLine	
Experimental Factor Type	strain_or_line	
Experimental Factor Term Source REF	
Person Last Name	Hummel	
Person First Name	Oliver	
Person Mid Initials	
Person Email	o.hummel@mdc-berlin.de	
Person Phone	
Person Fax	
Person Address	Prof. Hubner, Experimental Genetics of Cardiovascular Diseases, Max-Delbrück-Center,  , Berlin, 13125, Germany	
Person Affiliation	Max-Delbruck-Center	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	biological_replicate	
Quality Control Term Source REF	The MGED Ontology	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-04-15	
PubMed ID	
Publication DOI	
Publication Author List	Matthias Heinig, Enrico Petretto, Chris Wallace, Leonardo Bottolo, Maxime Rotival,Han Lu, Yoyo Li, Rizwan Sarwar, Sarah R. Langley, Anja Bauerfeind, Oliver Hummel, Young-Ae Lee, Svetlana Paskas, Carola Rintisch, Kathrin Saar, Jason Cooper, Rachel Buchan, Elizabeth E. Gray, Jason G. Cyster, Jeanette Erdmann, Christian Hengstenberg, Seraya Maouche, Willem H. Ouwehand, Catherine M. Rice, Nilesh J. Samani,  Heribert Schunkert, Alison H. Goodall, Herbert Schulz, Helge G. Roider, Martin Vingron, Stefan Blankenberg, Thomas Munzel, Tanja Zeller, Silke Szymczak, Andreas Ziegler, Laurence Tiret, Deborah J. Smyth, Michal Pravenec, Timothy J. Aitman, Francois Cambien, David Clayton, John A. Todd, Norbert Hubner & Stuart A. Cook	
Publication Title	A trans-acting locus regulates an anti-viral expression network and type 1 diabetes risk	
Publication Status	
Publication Status Term Source REF	
Experiment Description	We aimed to identify gene expression profiles/differences between spontaneously hypertensive rat(SHR) and normotensive rat (BN) in a set of recombinant inbred (RI) strains.	
Protocol Name	P-TABM-2517	P-TABM-2516	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	P-TABM-2518	
Protocol Type	grow	nucleic_acid_extraction	hybridization	feature_extraction	bioassay_data_transformation	
Protocol Description	Prague-Rats. Rats were housed in an air-conditioned animal facility and allowed free access to standard laboratory chow and water. All experiments were performed in agreement with the Animal Protection Law of the Czech Republic (311/1997) and were approved by the Ethics Committee of the Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.	Whole snap-frozen pulverized tissues were stored in aliquots at ﾖ80ï¾°C for RNA experiments. Total RNA was extracted using Trizol reagent (Invitrogen) and purified using RNeasy Mini kit (Qiagen) in accordance with the manufacturer's protocol.	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	RMA (background adjustment, quantile normalization, median polish as summarization method)	
Protocol Parameters	
Protocol Hardware	
Protocol Software			MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF			mo		The MGED Ontology	
SDRF File	E-TABM-458.sdrf.txt	
Term Source Name	ma	ncbitax	EFO	The MGED Ontology	ArrayExpress	The MGED Ontology	mo	EFO	
Term Source File	http://berkeleybop.org/cgi-bin/obofoundry/detail.cgi?id=adult_mouse_anatomy	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	
Term Source Version