Comment[ArrayExpressAccession]	E-MTAB-674
Investigation Title	Transcriptional profiling of Dicer conditional knockout hematopoietic stem cells.
Comment[Submitted Name]	Transcriptional profiling of Dicer conditional knockout hematopoietic stem cells.
Experiment Description	Gene expression of HSC  (Lin-Sca-Kit+Flt3-) hematopoietic stem cells from Dicer D/+ mice was compared with LT-HSC from DicerD/D mice.  BM cells were noncompetitively transplanted into irradiant recipients, and deletion induced by poly(IC) injection.  Donor-derived HSCs were isolated by FACS sorting of recipient bone marrow.  Biological replicates of each (2 per genotype) were generated and expression profiles were determined by hybridization to Affymetrix Moe430_2 arrays.
Experimental Design	genetic_modification_design
Comment[AEExperimentType]	transcription profiling by array
Experimental Factor Name	Genotype
Experimental Factor Type	Genotype
Quality Control Type	biological_replicate
Public Release Date	2012-09-21
Person Last Name	Nerlov
Person First Name	Claus
Person Mid Initials
Person Email	cnerlov@staffmail.ed.ac.uk
Person Phone	+44 131 650 5853
Person Address	University of Edinburgh, Kings Buildings, West Mains Road, Edinburgh EH9 3JQ
Person Affiliation	MRC Centre for Regenerative Medicine, Institute for Stem Cell Research
Person Roles	submitter
PubMed ID	22869792
Publication Author List	Natalija Buza-Vidas; Valeriu B. Cismasiu; Susan Moore; Adam J. Mead; Petter S. Woll; Michael Lutteropp; Luca Melchiori; Sidinh Luc; Tiphaine Bouriez-Jones; Deborah Atkinson; Donal OCarroll; Sten Eirik W. Jacobsen; Claus Nerlov
Publication Title	Dicer is selectively important for the earliest stages of erythroid development
Publication Status
Protocol Name	P-MTAB-20642	P-MTAB-20643
Protocol Type	grow	nucleic_acid_extraction
Protocol Description	Donor-derived hematopoietic stem cells (LSKFlt3-) were FACS-sorted from bone marrow of noncompetitively transplanted recipient mice. 	Approximately 10^3 cells were lysed in RLT buffer (Qiagen).  Total RNA was isolated using an RNeasy Micro kit.
Term Source Name	ArrayExpress
Term Source File	http://www.ebi.ac.uk/arrayexpress
SDRF File	E-MTAB-674.sdrf.txt