Comment[ArrayExpressAccession]	E-MTAB-476
Investigation Title	BIOLAN
Comment[Submitted Name]	BIOLAN
Experiment Description	Identification  of expression profiles in colorectal cancer using tumoral and non-tumoral tissue samples
Experimental Design	disease_state_design
Comment[AEExperimentType]	transcription profiling by array
Experimental Factor Name	DISEASESTATE
Experimental Factor Type	disease_state
Quality Control Type	biological_replicate
Public Release Date	2011-01-11
Person Last Name	Garcia-Bilbao
Person First Name	Amaia
Person Email	garciaam@gaiker.es
Person Phone	34 946002323
Person Address	Parque tecnologico edificio 202, 48170 Zamudio, Basque Country (Spain)
Person Affiliation	GAIKER
Person Roles	submitter; investigator
PubMed ID	22280244
Publication Author List	Garcia-Bilbao A, Armananzas R, Ispizua Z, Calvo B, Alonso-Varona A, Inza I, Larranaga P, Lopez-Vivanco G, Suarez-Merino B, Betanzos M.
Publication Title	Identification of a biomarker panel for colorectal cancer diagnosis.
Publication Status
Protocol Name	P-MTAB-18600	P-MTAB-18601	P-MTAB-18602	P-MTAB-18603	P-MTAB-18604	P-MTAB-18605
Protocol Type	nucleic_acid_extraction	Pool preparation	labeling	labeling	hybridization	image_acquisition
Protocol Description	Total RNA was extracted using RNEasy mini kit (Qiagen). RNA was quantified using Agilent 2100 Bioanalyzer.	2 ug of 33 out of the 34 non tumoral samples were collected to form a pool. These samples were: M1, M5, M8, M10, M11, M14, M20, M25, M29, M35, M37, M41, M43, M49, M51, M53, M55, M60, M65, M71, M74, M78, M80, M83, M88, M90, M94, M97, M99, M107, M112, M114, M120. (Sample not listed in E-MTAB-476.sdrf.txt: Id = 67636, Age = 73; Sample name = M35; OrganismPart = non-tumoral; Sex = Male; DiseaseState = NT; Provider = Hospital Cruces)	Pool was Cy3-labeled using Low RNA Input linear amplification kit (Agilent 5184-3523)	non-tumoral and tumoral samples were Cy5-labeled using Low RNA Input linear amplification kit (Agilent 5184-3523)	750 ng of labelled samples were hybridized onto Human 1A Oligo Microarrays (22K) (Agilent) using Agilent in situ hybridization kit plus (5184-3568). We used G2534A hybridization chambers. Microarrays were hybridized for 17 hours in Agilent's Hybridization oven (G2545A).	We used Genepix 4000B scanner. Slides were scanned at 10 um of resolution
SDRF File	E-MTAB-476.sdrf.txt
Comment[AEExperimentDisplayName]	BIOLAN
Publication DOI	10.1186/1471-2407-12-43