MAGE-TAB Version	1.1
Investigation Title	RNA-Sequencing of wild type and UCP1-null beige adipose tissue

Experimental Design	genotype design	genetic modification design
Experimental Design Term Source REF	EFO	EFO
Experimental Design Term Accession Number	EFO_0001748	EFO_0001758

Experimental Factor Name	genetic modification	genotype
Experimental Factor Type	genetic modification	genotype
Experimental Factor Term Source REF	EFO	EFO
Experimental Factor Term Accession Number	EFO_0000510	EFO_0000513

Person Last Name	Shinoda
Person First Name	Kosaku
Person Mid Initials	
Person Email	kshinoda@diabetes.ucsf.edu
Person Phone	
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Person Affiliation	Diabetes Center, University of California, San Francisco (UCSF)
Person Address	
Person Roles	submitter
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Normalization Type
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Date of Experiment	2015-11-24
Public Release Date	2017-10-28

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Experiment Description	Since brown adipose tissue (BAT) dissipates energy through UCP1, BAT has garnered attention as a therapeutic intervention for obesity and metabolic diseases including type 2 diabetes. As we better understand the roles of classical brown and beige adipocytes, increased beige fat mass in response to a variety of external/internal cues is associated with significant improvements in glucose and lipid homeostasis that may not be entirely mediated by UCP1. We aim to analyze transcriptome of wild type and UCP1-null beige adipocyte to identify the UCP1-independent function.

Protocol Name	P-MTAB-47563	P-MTAB-47560	P-MTAB-47561	P-MTAB-47562
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid sequencing protocol	nucleic acid library construction protocol
Protocol Term Source REF	EFO	EFO	EFO	EFO
Protocol Term Accession Number	EFO_0005518	EFO_0002944	EFO_0004170	EFO_0004184
Protocol Description	inguinal white adipose tissue	RIBOZOL RNA EXTRACTION REAGENT	1X75 sequencing	Nugen Oviation Ultralow Library System
Protocol Parameters				
Protocol Hardware			NextSeq 500	
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Term Source Name	EFO	ArrayExpress
Term Source File	http://www.ebi.ac.uk/efo/	http://www.ebi.ac.uk/arrayexpress/
Term Source Version	2.38

SDRF File	E-MTAB-4085.sdrf.txt
Comment [Submitted Name]	RNA-Sequencing of wild type and UCP1-null beige adipose tissue
Comment [SecondaryAccession]	ERP013315
Comment [SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/ERR1146201-ERR1146212
Comment [AEExperimentType]	RNA-seq of coding RNA
Comment[ArrayExpressAccession]	E-MTAB-4085
Comment [AEMINSEQEScore]	2