Investigation Title Genotyping of human cancer cell lines Comment[Submitted Name] SNPs Genotyping for Cancer Cell Line Project Experimental Design disease_state_design co-expression_design genotyping by array Experimental Design Term Source REF The MGED Ontology The MGED Ontology EFO Comment[SecondaryAccession] woost-00035 Comment[ArrayExpressReleaseDate] 2008-11-17 Comment[AEMIAMESCORE] 4 Comment[ArrayExpressAccession] E-MTAB-38 Comment[MAGETAB TimeStamp_Version] 2011-08-22 20:57:03 Last Changed Rev: 14857 Experimental Factor Name CELLLINE BIOSOURCEPROVIDER CLINICALINFORMATION ORGANISMPART DISEASESTATE Experimental Factor Type cell_line BioSourceProvider clinical_information organism_part disease_state Experimental Factor Term Source REF Person Last Name Wooster Person First Name Richard Person Mid Initials F Person Email richard.f.wooster@gsk.com Person Phone 610-270-6551 Person Fax Person Address "709 Swedeland Road, King of Prussia, 19406" Person Affiliation Person Roles submitter Person Roles Term Source REF Quality Control Type Quality Control Term Source REF Replicate Type Replicate Term Source REF Normalization Type Normalization Term Source REF Date of Experiment Public Release Date 2008-11-17 PubMed ID Publication DOI Publication Author List Publication Title None Publication Status Publication Status Term Source REF Experiment Description Multiple genome-wide microarray 500K SNP studies performed to measure the genotype profile of various cancer cell lines Protocol Name P-MTAB-1056 P-MTAB-1057 P-MTAB-1058 P-MTAB-1059 P-AFFY-6 Protocol Type grow nucleic_acid_extraction labeling hybridization feature_extraction Protocol Description Cell Lines were plated and digested with restriction enzyme Sty or NSP "Digested DNA was ligated to an adaptor and amplified by PCR using Platinum Pfx DNA Polymerase, yeilding a product of 250-2000bp" Genechip DNA Labeling biotin-labeled reagent for end-labeling fragmented PCR amplicons - https://www.affymetrix.com/support/downloads/manuals/500k_assay_manual.pdf standard Affymetrix hybridization protocol - https://www.affymetrix.com/support/downloads/manuals/500k_assay_manual.pdf Title: Affymetrix CEL analysis. Description: Protocol Parameters Protocol Hardware Protocol Software MicroArraySuite 5.0 Protocol Contact Protocol Term Source REF SDRF File E-MTAB-38.sdrf.txt Term Source Name NCI Thesaurus efo.obo NCI_thesaurus ATCC mo EFO The MGED Ontology ArrayExpress The MGED Ontology EFO Term Source File http://nciterms.nci.nih.gov/NCIBrowser/Dictionary.do /nfs/ma/ma-exp/GEOImportDownload/efo.obo ncithesaurus.obo.alt http://www.atcc.org/ http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/efo/ http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/arrayexpress http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/efo/ Term Source Version