Comment[ArrayExpressAccession]	E-MTAB-2054
MAGE-TAB Version	1.1
Investigation Title	Genomewide analysis of hypoxia regulated genes in the maternal blood during parturition
Comment[Submitted Name]	Genomewide analysis of hypoxia regulated genes in the maternal blood during parturition
Experiment Description	Microarray of whole blood maternal samples comparing the expression of genes in the maternal blood in pregnancies where the fetus was hypoxic at birth compared to those where the fetus was normoxic at birth. Intrapartum hypoxia is associated with severe neonatal morbidity and mortality. Current techniques to assess fetal hypoxic status during labour have a poor sensitivity. At birth, the lactate level in umbilical cord blood is used to assess the degree of fetal hypoxia during birth, such that a high lactate reflects a hypoxic fetus and a low lactate a normoxic fetus. We collected maternal blood prior to delivery and compared the expression of genes in the maternal blood in women delivering a normoxic or hypoxic fetus as evidenced by umbilcal cord blood levels.
Experimental Design	stimulus_or_stress_design	in_vivo_design	co-expression_design
Comment[AEExperimentType]	transcription profiling by array
Comment[AEExperimentDisplayName]	Transcription profiling by array of maternal whole blood samples comparing gene expression in pregnancies where the fetus was hypoxic at birth compared to those where the fetus was normoxic at birth
Experimental Factor Name	fetal oxygen status
Experimental Factor Type	clinical information
Quality Control Type	biological replicate
Quality Control Term Source REF	EFO
Public Release Date	2014-05-08
Person Last Name	Whitehead
Person First Name	Clare
Person Mid Initials
Person Email	clarew@unimelb.edu.au
Person Phone
Person Address
Person Affiliation	University of Melbourne
Person Roles	submitter
PubMed ID
Publication Author List	Clare Whitehead, Wan T Teh, Susan P Walker, Cheryl Leung, Sonali Mendis, Luke Lamour and Stephen Tong
Publication Title	Quantifying circulating hypoxia-induced RNA transcripts in maternal blood to determine in utero fetal hypoxic status
Publication Status	Under review
Protocol Name	P-MTAB-35970	P-MTAB-35971	P-MTAB-35972	P-MTAB-35973	P-MTAB-35974	P-MTAB-35975
Protocol Type	Hypoxia	Extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning protocol	normalization protocol
Protocol Description	Maternal whole blood was collected in PAXgene tubes  from labouring women delivering hypoxic or normoxic fetuses	RNA was extracted using the PAXgene miRNA protocol as per manufacturer's instructions. Quality control wasperformed with the Agilent Bioanalyzer.	Labelling was performed by the Australian Genome Research Facility (AGRF) using the Ambion MessageAmp kit for Illumina	Standard Illumina hybridization protocol	Standard Illumina scanning protocol.	The data were normalized using limma in R.
Protocol Term Source REF	EFO	EFO	EFO	EFO	EFO	EFO
Term Source Name	EFO	ArrayExpress
Term Source File	http://www.ebi.ac.uk/efo	http://www.ebi.ac.uk/arrayexpress
SDRF File	E-MTAB-2054.sdrf.txt