Comment[ArrayExpressAccession]	E-MTAB-1504
MAGE-TAB Version	1.1
Investigation Title	gata1_ter119_submission_papadopoulos
Comment[Submitted Name]	gata1_ter119_submission_papadopoulos
Experiment Description	TODO
Experimental Design	development_or_differentiation_design	in_vivo_design	ChiP-seq
Comment[AEExperimentType]	ChIP-seq
Comment[AEExperimentDisplayName]	ChIP-seq of mouse fetal liver cells from E12.5 embryos to investigate GATA-1 genome-wide occupancy associates
Experimental Factor Name	immunoprecipitate	developmental stage
Experimental Factor Type	immunoprecipitate	developmental stage
Quality Control Type	biological_replicate
Public Release Date	2013-03-26
Person Last Name	Strouboulis
Person First Name	John
Person Mid Initials
Person Email	strouboulis@fleming.gr
Person Phone	+ 30 210 8970052
Person Address	16602 Varkiza, Greece
Person Affiliation	BSRC Alexander Fleming, Division of Molecular Oncology
Person Roles	submitter
PubMed ID	23519611
Publication Author List	Papadopoulos GL, Karkoulia E, Tsamardinos I, Porcher C, Ragoussis J, Bungert J, Strouboulis J
Publication Title	GATA-1 genome-wide occupancy associates with distinct epigenetic profiles in mouse fetal liver erythropoiesis
Publication Status	Under Review
Protocol Name	P-MTAB-31110	P-MTAB-31111	P-MTAB-31112	P-MTAB-31113	P-MTAB-31114	P-MTAB-31115
Protocol Type	grow	specified_biomaterial_action	nucleic_acid_extraction	sequencing	scanning	nucleic acid library construction protocol
Protocol Description	Fetal liver cells from day E12.5 C57/BL6 mouse embryos expanded for 3 days in serum free medium (von Lindern et al, 2001)	Fractionation of Ter119- and Ter119+ cells with Miltenyi beads (Schuh et al, 2005)	Formaldehyde-crosslinked chromatin from 10^7 Ter119- or Ter119+ fetal liver cells was prepared as previously described (Schuh et al, 2005)	Anti GATA-1 ChIPed DNA from Ter119-, Ter119+ and a 'no antibody' (input DNA) were processed for deep sequencing using the Illumina Genome Analyzer II platform according to Illumina protocols (www.illumina.com). Deep sequencing was carried out in duplicate for each ChIP sample and once for Input DNA controls producing 51-nuleotide sequence reads.	Sequencing was performed using an Illumina Genome Analyzer II platform. Single reads of 51bp were generated.	ChIPed and INPUT DNA libraries were constructed according to Illumina protocols (http://www.illumina.com).
Protocol Hardware				Illumina Genome Analyzer II
Comment[SecondaryAccession]	ERP002249
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/ERR231633-ERR231638
SDRF File	E-MTAB-1504.sdrf.txt
Publication DOI	10.1093/nar/gkt167