Comment[ArrayExpressAccession]	E-MTAB-1496
Investigation Title	The Ludwig Institute for Cancer Research Melbourne Melanoma Cell Line Panel																	
Comment[Submitted Name]	The Ludwig Institute for Cancer Research Melbourne Melanoma Cell Line Panel
Experiment Description	Expression profiling of a panel of metastatic melanoma cell lines																	
Experimental Design	cell_type_comparison_design	in_vitro_design																
Comment[AEExperimentType]	transcription profiling by array																	
Comment[AEExperimentDisplayName]	Transcription profiling by array of human melanoma cell lines to investigate gene expression																	
Experimental Factor Name	cell line																	
Experimental Factor Type	cell line																	
Quality Control Type																		
Public Release Date	2013-02-01																	
Person Last Name	Anaka																	
Person First Name	Matthew																	
Person Mid Initials																		
Person Email	matthew.anaka@ludwig.edu.au																	
Person Phone	+613 9496 5726																	
Person Address	Ludiwg Institute for Cancer Research																	
Person Affiliation	Melbourne Austin Branch, Austin Hospital HSB6, 145-163 Studley Road, Heidelberg, Victoria, 3084, Australia																	
Person Roles	submitter																	
PubMed ID																		
Publication Author List																		
Publication Title																		
Publication Status																		
Protocol Name	P-MTAB-21676	P-MTAB-21678	P-MTAB-31024	P-MTAB-31025	P-MTAB-31026
Protocol Type	grow	nucleic_acid_extraction	labeling	hybridization	image_acquisition													
Protocol Description	RF10 consists of basal RPMI 1640 (Invitrogen) with 2mM Glutamax (Invitrogen), 100U/ml Penicillin (Invitrogen),  and 100ug/mL Streptomycin (Invitrogen), and 10% foetal calf serum (CSL, Melbourne, Australia). (Parameters: time unit = seconds, temperature un	RNA was extracted along with gDNA and protein using Qiagen AllPrep kits. (Parameters: Extracted product = total_RNA, Amplification = none)	ManufacturerM-^Rs (Illumina) standard protocol - 200ng starting material	ManufacturerM-^Rs (Illumina) standard protocol - 48C for 17 hours	Beadchips were scanned using a Illumina Beadstation 500GX using default settings													
SDRF File	E-MTAB-1496.sdrf.txt