Comment[ArrayExpressAccession]	E-MTAB-1449
Investigation Title	renal damage in 24w2k1c  v cont24w
Comment[Submitted Name]	renal damage in 24w2k1c  v cont24w
Experiment Description	The experiment was designed to study the effects of blood pressure on gene expressions in the kidney cortex. Non-clipped kidney cortex was harvested from eight 2k1c rats and eight sham rats after 4 and 24 weeks of clipping. The 2k1c is the experimental group which underwent a two kidney, one clip (2k1c) operation (renal artery stenosis), wheras the sham group serves as a control where no stenosis were performed.
Experimental Design	in_vitro_design	co-expression_design	injury design
Comment[AEExperimentType]	transcription profiling by array
Comment[AEExperimentDisplayName]	Transcription profiling by array of kidney cortex harvested from 2k1c and sham rats to study the effects of blood pressure on gene expression in the kidney cortex
Experimental Factor Name	TREATMENT	AGE
Experimental Factor Type	treatment	age
Quality Control Type	biological replicate
Public Release Date	2014-11-11
Person Last Name	Skogstrand
Person First Name	Trude
Person Mid Initials
Person Email	trude.skogstrand@med.uib.no
Person Phone	4755972959
Person Address	University of Bergen, Department of Medicine
Person Affiliation
Person Roles	submitter;investigator
PubMed ID	PMID: 25380151
Publication Author List	Skogstrand T, Leh S, McClure J, Dashti M, Iversen BM, Graham D, McBride MW, Hultström M.
Publication Title	Identification of a common molecular pathway in hypertensive renal damage: comparison of rat and human gene expression profiles.
Publication Status
Protocol Name	P-MTAB-30588	P-MTAB-30589	P-MTAB-30590	P-MTAB-30591	P-MTAB-30592	P-MTAB-30593
Protocol Type	specified_biomaterial_action	nucleic_acid_extraction	labeling	hybridization	image_acquisition	bioassay_data_transformation
Protocol Description	Rats were 2k1c or sham operated at 6 weeks of age. Rats were sacrificed at 4 weeks and 24 weeks after the operation. Rats were killed under isoflurane anaesthesia, the kidneys were flushed with ice cold PBS and a slice was placed directly into RNA-later.	RNA was extracted from kidney tissue after homogenisation using RN-Easy	Illunima TotalPrep RNA amplification kit	Whole-Genome Gene Expression Direct Hybridization Assay Guide, Illumina Inc. 750ng were hybridized at 58 degrees for 18.5 hours on a rocker.	Illumina iScan Reader with bead-level output.	normaliseIllumina(X, method=quantile, transform=log2)
SDRF File	E-MTAB-1449.sdrf.txt
Publication DOI