Comment[ArrayExpressAccession]	E-MTAB-1423
MAGE-TAB Version	1.1
Investigation Title	ChIP-seq on p300, NFI and H3K27ac in mouse neural stem cells
Comment[Submitted Name]	ChIP-seq on p300, NFI and H3K27ac in mouse neural stem cells
Experiment Description	Definition of genome wide transcriptional enhancers by overlap of p300 and H3K27ac and the role of NFI factors in the regulation of mouse neural stem cells
Experimental Design	growth_condition_design	in_vitro_design	ChiP-seq
Comment[AEExperimentType]	ChIP-seq
Comment[AEExperimentDisplayName]	ChIP-seq of mouse neural stem cells cultuered in either proliferation-promoting medium (proNSC) or quiescence-promoting medium (quiNSC) on p300, Nuclear factor-I (NFI) and acetylated H3K27 (H3K27ac)
Experimental Factor Name	immunoprecipitate	growth condition
Experimental Factor Type	immunoprecipitate	growth condition
Public Release Date	2013-08-12
Person Last Name	Mateo	Martynoga	Guillemot
Person First Name	Juan	Ben	Francois
Person Mid Initials	L.
Person Email	juan.mateo@cos.uni-heidelberg.de	bmartyn@nimr.mrc.ac.uk	fguille@nimr.mrc.ac.uk
Person Phone	0049 (0)6221 54 6493	0044 (0)2088162497	0044 (0)2088162470
Person Address	Im Neuenheimer Feld 230, 69120 Heidelberg, Germany	National Institute for Medical Research, Mill Hill, London NW7 1AA, UK	National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
Person Affiliation	Centre for Organismal Studies (COS) - University of Heidelberg	Division of Molecular Neurobiology, National Institute for Medical Research	Division of Molecular Neurobiology, National Institute for Medical Research
Person Roles	submitter	primary author	corresponding author
Protocol Name	P-MTAB-30250	P-MTAB-30251	P-MTAB-30252	Illumina Genome Analyzer IIx
Protocol Type	growth protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol
Protocol Description	Neural stem cells cultured in either proliferation-promoting medium (proNSC) or quiescence-promoting medium (quiNSC)	Chromatin Immunoprecipitation with antibodies to H3K27ac (Abcam, ab4729), p300 (Santa Cruz, sc-585) and NFI transcription factors (Santa Cruz, sc-30918).	Libraries were prepared from 10ng of ChIP-enriched DNA using Illumina's ChIP-seq DNA sample preparation kit. This was sequenced to generate single end 40bp reads.	ILLUMINA sequencing using Illumina Genome Analyzer IIx. http://www.illumina.com/support/sequencing/sequencing_instruments.ilmn
Protocol Hardware				Illumina Genome Analyzer IIx
Term Source Name	ArrayExpress
Term Source File	http://www.ebi.ac.uk/arrayexpress
Comment[SecondaryAccession]	ERP002084
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/ERR216106-ERR216112
SDRF File	E-MTAB-1423.sdrf.txt
PubMed ID	23964093
Publication Author List	Ben Martynoga; Juan L. Mateo; Bo Zhou; Jimena Andersen; Angeliki Achimastou; Noelia Urban; Debbie van den Berg; Dimitra Georgopoulou; Suzana Hadjur; Joachim Wittbrodt; Laurence Ettwiller; Michael Piper; Richard M. Gronostajski; Francois Guillemot
Publication Title	Epigenomic enhancer annotation reveals a key role for NFIX in neural stem cell quiescence