Comment[ArrayExpressAccession] E-MTAB-1404 Investigation Title Ring1b profiling in pancreatic islets Comment[Submitted Name] Ring1b profiling in pancreatic islets Comment[AEExperimentDisplayName] Transcription profiling by array of mouse pancreatic islets from mice with pancreatic conditional Ring1b homozygous knock-out vs littermate controls Comment[AEExperimentType] transcription profiling by array Experimental Design organism_part_comparison_design in_vivo_design Experiment Description To gain insights into how pancreatic cells are programmed in vivo, we profiled RNA expression in pancreatic islets of pancreatic Ring1b conditional KO mice (conditional using a pancreas specfic Cre; Pdx1-Cre) and their littermate controls Experimental Factor Name genotype Experimental Factor Type genotype Person Last Name van arensbergen Person First Name joris Person Email joris.van.arensbergen@gmail.com Person Address Plesmanlaan 121, 1066CX, amsterdam Person Affiliation Netherlands Cancer Institute, Division of gene regulation Person Roles submitter Quality Control Type biological_replicate Public Release Date 2013-02-10 Protocol Name P-MTAB-30036 P-MTAB-30037 P-MTAB-30038 Protocol Type treatment nucleic_acid_extraction labeling Protocol Description Mouse pancreatic islets were isolated as described in (Luco et al., 2008) Total RNA was extracted from pancreatic islet preparations of 3 individual male animals for each genotype using Trizol (Invitrogen). RNA integrity was verified with a 2100 Bioanalyzer (Agilent), and labeled cRNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. Term Source Name ArrayExpress Term Source File http://www.ebi.ac.uk/arrrayexpress SDRF File E-MTAB-1404.sdrf.txt Publication Title Ring1b bookmarks genes in pancreatic embryonic progenitors for repression in adult β cells. Publication Author List van Arensbergen J, García-Hurtado J, Maestro MA, Correa-Tapia M, Rutter GA, Vidal M, Ferrer J PubMed ID 23271347 Publication DOI 10.1101/gad.206094.112