Comment[ArrayExpressAccession]	E-MTAB-11
Comment[AdditionalFile:XLS]	E-MTAB-11.hybs.xls
Investigation Title	Transcription profiling of peripheral blood mononuclear cells taken from rheumatoid arthritis patients before and after treatment with Ethanercept (Enbrel)
Comment[Submitted Name]	Enbrel therapy monitoring
Publication Title	Arthritis Res Ther
Publication Author List	Koczan D, Drynda S, Hecker M, Drynda A, Guthke R, Kekow J, Thiesen HJ.
Publication DOI
PubMed ID	18454843
Experimental Design	in_vivo_design	co-expression_design	time_series_design	compound_treatment_design
Experimental Design Term Source REF		The MGED Ontology	The MGED Ontology	The MGED Ontology
Comment[MIAMExpressLogin]	Koczan
Comment[MIAMExpressSubmissionID]	4235
Experiment Description	Rheumatoid Arthritis patients were with Ethanercept (Enbrel), blocking the TNF alpha signalcascade by receptor mimicri. Peripheral blood samples were taken at 3 timepoints, before the first and 2 next drug injections after 3 and 6 days (t0, t1 (72h), t2 (144h)). Peripheral blood mononuclear cells were isolated by standard Ficoll gradient centrifugation. Whole RNA samples were profiled on Affymetrix Human Genome U95A and Human Genome U133A arrays. Later time point were analysed against timepoint 0 and differential gene expression signatures were correlated to clinical data i.e. responsiveness. <br><br>This experiment was reloaded into ArrayExpress on 27th January 2010 to fix errors in the CEL and CHP file association with hybridizations.
Experimental Factor Name	CLINICAL_INFORMATION	COMPOUND	TIME_POINT	TIME
Experimental Factor Type	clinical_information	compound	timepoint	time
Person Last Name	Koczan
Person First Name	Dirk
Person Email	dirk.koczan@med.uni-rostock.de
Person Phone	+49 381 4945885
Person Fax	+49 381 4945882
Person Affiliation	University of Rostock
Person Address	Schillingallee 70, Rostock, MVP, 18055, Germany
Person Roles	submitter
Person Roles Term Source REF	MGED Ontology
Public Release Date	2010-04-22
Term Source Name	MGED Ontology		The MGED Ontology	EFO
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php			http://efo.svn.sourceforge.net/viewvc/efo/trunk/src/efoinowl/efo.owl
Protocol Name	P-MTAB-74
Protocol Type	nucleic_acid_extraction
Protocol Description	Peripheral blood monocytes were isolated using standard Ficoll gradient centrifugation. The cells were lysed in RLT buffer/1% ME(Qiagen Hilden). Whole RNA extraction was performed according to manufactures protocol.
Protocol Term Source REF	MGED Ontology
SDRF File	E-MTAB-11.sdrf.txt